Journal: bioRxiv

Article Title: Activity of Antibiotics against Burkholderia cepacia complex in Artificial Sputum Medium

doi: 10.1101/2023.12.13.571502

Figure Lengend Snippet: Time-kill studies in ASMDM with established growth of (a) B. cenocepacia K56-2 and (b) B. multivorans CGD1 MIN: minocycline; MER: meropenem; TOB: tobramycin; LVX: levofloxacin; T/S: trimethoprim-sulfamethoxazole; CAZ: ceftazidime. Graphs show geometric mean and standard deviation of ≥3 replicates. Dashed line indicates assay lower limit of detection. For assays performed simultaneously, the same growth control wells were used.

Article Snippet: Stocks of minocycline and ceftazidime (Chem-Impex Intl., Wood Vale, IL), meropenem (GoldBio, St. Louis, MO), tobramycin (Research Products Intl., Mt.

Techniques: Standard Deviation, Control

Journal: bioRxiv

Article Title: Activity of Antibiotics against Burkholderia cepacia complex in Artificial Sputum Medium

doi: 10.1101/2023.12.13.571502

Figure Lengend Snippet: Time-kill studies in different conditions: B. cenocepacia K56-2 ASMDM: Artificial sputum medium; CAMHB: cation-adjusted Mueller Hinton broth; MIN: minocycline; MER: meropenem; TOB: tobramycin; LVX: levofloxacin; T/S: trimethoprim-sulfamethoxazole; CAZ: ceftazidime. Graphs show geometric mean and standard deviation of ≥3 replicates. Dashed line indicates assay lower limit of detection. For assays performed simultaneously, the same growth control wells were used.

Article Snippet: Stocks of minocycline and ceftazidime (Chem-Impex Intl., Wood Vale, IL), meropenem (GoldBio, St. Louis, MO), tobramycin (Research Products Intl., Mt.

Techniques: Standard Deviation, Control

Journal: bioRxiv

Article Title: Activity of Antibiotics against Burkholderia cepacia complex in Artificial Sputum Medium

doi: 10.1101/2023.12.13.571502

Figure Lengend Snippet: Time-kill studies in different conditions: B. multivorans CGD1 ASMDM: Artificial sputum medium; CAMHB: cation-adjusted Mueller Hinton broth; MIN: minocycline; MER: meropenem; TOB: tobramycin; LVX: levofloxacin; T/S: trimethoprim-sulfamethoxazole; CAZ: ceftazidime. Graphs show geometric mean and standard deviation of ≥3 replicates. Dashed line indicates assay lower limit of detection. For assays performed simultaneously, the same growth control wells were used.

Article Snippet: Stocks of minocycline and ceftazidime (Chem-Impex Intl., Wood Vale, IL), meropenem (GoldBio, St. Louis, MO), tobramycin (Research Products Intl., Mt.

Techniques: Standard Deviation, Control

Journal: bioRxiv

Article Title: Activity of Antibiotics against Burkholderia cepacia complex in Artificial Sputum Medium

doi: 10.1101/2023.12.13.571502

Figure Lengend Snippet: Metabolic activity (heat flow) of bacteria grown in ASMDM for 72 hours prior to addition of antibiotics. (a) B. cenocepacia K56-2; (b) B. multivorans CGD1. MIN: minocycline; MER: meropenem; TOB: tobramycin; LVX: levofloxacin; T/S: trimethoprim-sulfamethoxazole; CAZ: ceftazidime. Concentrations are in μg/mL. T/S concentrations are expressed as the sum of the two components.

Article Snippet: Stocks of minocycline and ceftazidime (Chem-Impex Intl., Wood Vale, IL), meropenem (GoldBio, St. Louis, MO), tobramycin (Research Products Intl., Mt.

Techniques: Activity Assay, Bacteria

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: MicroRNA-186 targets SKP2 to induce p27 Kip1 -mediated pituitary tumor cell cycle deregulation and modulate cell proliferation

doi: 10.4196/kjpp.2019.23.3.171

Figure Lengend Snippet: (A, B) SKP2 expression in rat GH3 and human GH-secreting PA cells was regulated by transfection with si-SKP2 or SKP2 overexpressing vectors, as confirmed by immunoblot assays. Negative control small RNA interference (si-NC) or an empty vector (NC) served as a control. (C, D) Rat GH3 and human GH-secreting PA cells were transfected with si-SKP2 or SKP2 overexpressing vectors, and then DNA synthesis was examined using BrdU assays. (E, F) Cell proliferation was determined using CCK-8 assays. (G) Rat GH3 and human GH-secreting PA cells were cotransfected with si-SKP2 and si-p27 Kip1 , and then cell cycle analysis was performed using flow cytometer assays. The data are presented as the mean ± standard deviation of three independent experiments. * p < 0.05 and ** p < 0.01, compared to the si-NC or SKP2 overexpression vector group. si, small interfering; NC, negative control; GH, growth hormone; PA, pituitary adenoma; BrdU, 5-bromo-2′-deoxyuridine; OD, optical density.

Article Snippet: The rat pituitary tumor cell line GH3 and the human embryonic kidney epithelial cell line HEK293 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Expressing, Transfection, Western Blot, Negative Control, Plasmid Preparation, Control, DNA Synthesis, CCK-8 Assay, Cell Cycle Assay, Flow Cytometry, Standard Deviation, Over Expression

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: MicroRNA-186 targets SKP2 to induce p27 Kip1 -mediated pituitary tumor cell cycle deregulation and modulate cell proliferation

doi: 10.4196/kjpp.2019.23.3.171

Figure Lengend Snippet: (A) Rat GH3 and human GH-secreting PA cells were transfected with miR-186 mimics or inhibitors to regulate miR-186 expression, as confirmed using real-time PCR. (B) SKP2 mRNA expression in response to miR-186 overexpression or inhibition in rat GH3 and human GH-secreting PA cells was examined using real-time PCR. (C) SKP2 protein levels under the same conditions were examined using immunoblot assays. (D) Luciferase reporter gene assays were performed using wt-SKP2 3′-UTR or mut-SKP2 3′-UTR vectors to verify the direct binding between miR-186 and SKP2 3′-UTR. The data are presented as mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01. miR, microRNA; mRNA, messenger RNA; GH, growth hormone; PA, pituitary adenoma; PCR, polymerase chain reaction; UTR, untranslated region; NC, negative control.

Article Snippet: The rat pituitary tumor cell line GH3 and the human embryonic kidney epithelial cell line HEK293 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction, Over Expression, Inhibition, Western Blot, Luciferase, Binding Assay, Standard Deviation, Polymerase Chain Reaction, Negative Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: MicroRNA-186 targets SKP2 to induce p27 Kip1 -mediated pituitary tumor cell cycle deregulation and modulate cell proliferation

doi: 10.4196/kjpp.2019.23.3.171

Figure Lengend Snippet: (A) Rat GH3 and human GH-secreting PA cells were transfected with miR-186 mimics or inhibitors, and then, DNA synthesis was examined using BrdU assays. (B) Cell proliferation using CCK-8 assays. (C) Cell cycle analysis using flow cytometer assays. (D, E) Rat GH3 and human GH-secreting PA cells were cotransfected with miR-186 mimics and SKP2 overexpressing vectors, and then examined for the protein levels of SKP2 and p27 Kip1 were examined using Immunoblot assays. (F) DNA synthesis was examined using BrdU assays. (G) Cell proliferation was examined using CCK-8 assays. The data are presented as the mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, compared to the control group; ## p < 0.01, compared to the SKP2 group. miR, microRNA; GH, growth hormone; PA, pituitary adenoma; BrdU, 5-bromo-2′-deoxyuridine; UTR, untranslated region; NC, negative control; OD, optical density.

Article Snippet: The rat pituitary tumor cell line GH3 and the human embryonic kidney epithelial cell line HEK293 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Transfection, DNA Synthesis, CCK-8 Assay, Cell Cycle Assay, Flow Cytometry, Western Blot, Standard Deviation, Control, Negative Control

Journal: The BMJ

Article Title: Drug treatments for covid-19: living systematic review and network meta-analysis

doi: 10.1136/bmj.m2980

Figure Lengend Snippet: Randomised trials identified after data analysis, which will be included in the next update

Article Snippet: The 95% credible interval for all other interventions included no effect, and certainty was low or very low for all interventions except remdesivir (ratio of mean days between intervention and standard care, 0.82, 0.64 to 1.06; moderate certainty) ( ).

Techniques: